The cemetery is located near the eponymous Church of St. Wenceslas, on the place of an abondoned settlement called Lom. It was established in the 13th/14th century, when Bavor III founded the Church of St. Wenceslas with presbytery in the then village. In later become the only cemetery in Strakonice. Its ancient origin can be seen in the 16th century tombstones placed in the Church's floor. The grounds have always been consecrated. First it spread close to the church, but later it was extended to its present size.

It used to be a denominational cemetery. The patronage right over the cemetery and the church was first held by the grand Maltese priory, and later with the party cooperative that employed an undertaker and was responsible for order and maintenance. The Deacon's Office had final word with respect to the cult. After 1948 patronage was transferred to the state. The cemetery is presently operated by Technical Services Strakonice, Ltd.

The entrance to the cemetery is through a gate with figural ornaments. Here we can see the Holy Trinity Chapel (tomb of hereditary postmasters), the Adalbert of Prague Chapel, the high stone cross of 1888, the grave of Jan Vlastislav Plánek, and the crypt of the Papež family with a bust by Břetislav Benda.